Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Superior anti-tumor protection and therapeutic efficacy of vaccination with allogeneic and semiallogeneic dendritic cell/tumor cell fusion hybrids for murine colon adenocarcinoma

doi: 10.1007/s00262-006-0252-5

Figure Lengend Snippet: Induction of CTL or NK cell responses in immunized mice. Balb/c mice were immunized on days 0 and 7 with PBS only (open circle), syn-DC fusion (filled triangle), allo-DC fusion (filled circle) or semiallo-DC fusion (filled square). On day 14, immunized mouse were incubated with a CT26CL25, b CT26, c D5LacZ or d YAC-1 target cells at the ratios indicated. CTL activity, depicted as percent cytolysis, was determined using standard 4-h 51Cr release cytotoxity assays. Similar results were obtained in three separate experiments

Article Snippet: Cell lines The murine colon adenocarcinoma cell lines Balb/c-derived CT26 and CT26CL25 [CT26 stably transduced with the retroviral vector LXSN expressing β-galactosidase (β gal) as a model tumor-associated antigen (TAA)] were purchased from ATCC (Rockville, MD, USA).

Techniques: Incubation, Activity Assay

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Superior anti-tumor protection and therapeutic efficacy of vaccination with allogeneic and semiallogeneic dendritic cell/tumor cell fusion hybrids for murine colon adenocarcinoma

doi: 10.1007/s00262-006-0252-5

Figure Lengend Snippet: Anti-tumor therapeutic efficacy of each fusion vaccination. Balb/c mice were immunized with PBS only, syn-DC, allo-DC or semiallo-DC fusions on days 3 and 10 after intravenous inoculation of 5.0 × 105 CT26CL25 viable cells (day 0). a On day 24, animals were sacrificed and their lungs were stained with India Black Ink to count the number of pulmonary metastases (white nodules). b For each vaccinatioin group, the mean (+SD) numbers of pulmonary metastases were plotted (n = 5). *P < 0.001 (syn-DC fusion vs PBS control, allo-DC fusion vs syn-DC fusion). **P < 0.001 (semiallo-DC fusion vs syn-DC fusion) and P = 0.11 (semiallo-DC fusion vs. allo-DC fusion)

Article Snippet: Cell lines The murine colon adenocarcinoma cell lines Balb/c-derived CT26 and CT26CL25 [CT26 stably transduced with the retroviral vector LXSN expressing β-galactosidase (β gal) as a model tumor-associated antigen (TAA)] were purchased from ATCC (Rockville, MD, USA).

Techniques: Drug discovery, Staining, Control

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Superior anti-tumor protection and therapeutic efficacy of vaccination with allogeneic and semiallogeneic dendritic cell/tumor cell fusion hybrids for murine colon adenocarcinoma

doi: 10.1007/s00262-006-0252-5

Figure Lengend Snippet: Induction of AH-1 and TPH specific CTLs by each vaccine. Balb/c mice were immunized with PBS only, syngeneic DC/TC, allogeneic DC/TC or semiallogeneic DC/TC hybrids on days 0 and 7. TCs were either CT26CL25 (a) or CT26 cells (b). On day 14, splenocytes from immunized mice were incubated with irradiated CT26CL25 cells in the presence of both IL-2 and IL-7 for 3 days, and then assayed for pentameric assays using H-2Ld-AH-1 or H-2Ld-TPH pentamers

Article Snippet: Cell lines The murine colon adenocarcinoma cell lines Balb/c-derived CT26 and CT26CL25 [CT26 stably transduced with the retroviral vector LXSN expressing β-galactosidase (β gal) as a model tumor-associated antigen (TAA)] were purchased from ATCC (Rockville, MD, USA).

Techniques: Incubation, Irradiation

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Superior anti-tumor protection and therapeutic efficacy of vaccination with allogeneic and semiallogeneic dendritic cell/tumor cell fusion hybrids for murine colon adenocarcinoma

doi: 10.1007/s00262-006-0252-5

Figure Lengend Snippet: Anti-tumor protection mediated by each fusion vaccination. Balb/c mice were immunized with PBS only, syn-DC, allo-DC or semiallo-DC fusions on days 0 and 7 followed by subcutaneous inoculation of a 1.0 × 106 or b 5.0 × 106 CT26CL25 viable cells one week after the last immunization (day 14). The size of tumor (long × short diameter) in each mouse on day 35 was plotted. Level lines mark the average size within each group (n = 4)

Article Snippet: Cell lines The murine colon adenocarcinoma cell lines Balb/c-derived CT26 and CT26CL25 [CT26 stably transduced with the retroviral vector LXSN expressing β-galactosidase (β gal) as a model tumor-associated antigen (TAA)] were purchased from ATCC (Rockville, MD, USA).

Techniques:

Journal: Pathology Oncology Research

Article Title: Identification of Differentially Expressed Proteins in the Serum of Colorectal Cancer Patients Using 2D-DIGE Proteomics Analysis

doi: 10.1007/s12253-015-9991-y

Figure Lengend Snippet: Differentially expressed proteins identified by LC-MS/MS after DIGE analysis

Article Snippet: Human apolipoprotein A1 (APOA1), apolipoprotein E (APOE), complement factor H (CFH), synaptojanin-2 (SYNJ2) and galectin-7 (GAL7) were validated using ELISA kits (Cusabio Biotech, USA) according to the manufacturer’s protocols.

Techniques: Sequencing, Molecular Weight, Control, Retroviral, Coagulation, Transduction

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Retroviral vectors encoding a reverse transcription-activated transgene efficiently limit expression of the gene to target cells.

doi: 10.1038/sj.mt.6300062

Figure Lengend Snippet: Figure 2 Physical approximation of the reverse-oriented SV40- promoter and GFP gene in integrated proviruses. (a) Southern blot of genomic DNA from G418-selected PG13 producer and TE671 target cells, digested with StuI and HindIII, and hybridized with a 32P-labeled GFP-specific cDNA probe. Sample/lane identities are indicated along the top. Bands are observed where the SV40 promoter (StuI site) has been copied via reverse transcription to the 30LTR, i.e., in the TE671 cells transduced with RET and RETdc vectors. Bands are not detected in the corresponding PG13 producer cells or TE671 target cells trans- duced with retG or GLEN. Proviral structures with relevant restriction sites and expected band sizes are indicated at the bottom. (b) PCR of genomic DNA from the same samples used for Southern analysis using oligonucleotides specific for the GFP gene and the SV40 promoter. Reactions were run on a 1.5% agarose gel stained with ethidium bromide (EtBr). Sample/lane identities are as in a. Successful amplifica- tion requires the copying of the reverse-oriented SV40 promoter to the 30LTR. Appropriately sized PCR bands were obtained from TE671 cells transduced with the RET and RETdc vectors, but from no other samples. The greater intensity of the bands for the RETdc compared with the RET-transduced cells is likely the result of the second copy of the SV40-GFP cassette. Arrows in the vector diagrams indicate the location and orientation of the PCR primers. (c) Control PCR performed on the same genomic DNA samples using primers specific for 18S ribosomal RNA.

Article Snippet: PG13 gibbon ape leukemia virus (GaLV)-pseudotyped retroviral vector packaging cells39 were obtained from ATCC (Manassas, VA) and TE671 human rhabdomyosarcoma cells40 were a kind gift of Dr BA Bunnell (NHGRI, Bethesda, MD).

Techniques: Southern Blot, Labeling, Reverse Transcription, Transduction, Agarose Gel Electrophoresis, Staining, Plasmid Preparation, Control

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Retroviral vectors encoding a reverse transcription-activated transgene efficiently limit expression of the gene to target cells.

doi: 10.1038/sj.mt.6300062

Figure Lengend Snippet: Figure 3 Analysis of GFP mRNA expression in producer and target cells. The upper panel shows a Northern blot of total RNA from G418- selected PG13 producer and TE671 target cells hybridized with a 32P-labeled GFP-specific cDNA probe. Sample/lane identities are indicated along the top. In TE671 cells transduced with the RET and RETdc vectors, copying of the SV40 promoter to the 30LTR by reverse transcription resulted in the generation of a small (B1 kb) GFP-encoding transcript not seen in the corresponding PG13 producer cells or in TE671s transduced with retG or GLEN. Vector diagrams and expected transcripts are shown in Figure 1. Abbreviations: V: RET, retG, and RETdc genomic RNAs; VS: RET, retG, and RETdc spliced vector RNAs; VGLEN: GLEN genomic and spliced vector RNAs (indistinguishable); GFP: GFP spliced RNAs. Lower panel shows a control Northern blot of the same RNA samples using a 32P-labeled oligonucleotide probe specific for 18S ribosomal RNA.

Article Snippet: PG13 gibbon ape leukemia virus (GaLV)-pseudotyped retroviral vector packaging cells39 were obtained from ATCC (Manassas, VA) and TE671 human rhabdomyosarcoma cells40 were a kind gift of Dr BA Bunnell (NHGRI, Bethesda, MD).

Techniques: Expressing, Northern Blot, Labeling, Transduction, Reverse Transcription, Plasmid Preparation, Control

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Retroviral vectors encoding a reverse transcription-activated transgene efficiently limit expression of the gene to target cells.

doi: 10.1038/sj.mt.6300062

Figure Lengend Snippet: Figure 5 Flow cytometry analysis of GFP expression in producer and target cells. Dark shading shows the baseline fluorescence in untransduced cells. Light shading indicates expression in transfected producer or transduced target cells. PG13 cells were transfected with the indicated vectors, selected in G418, and the surviving colonies pooled. Supernatants were harvested and used to transduce TE671 cells, which were then selected in G418 and pooled. Analysis of the PG13 cells was performed at the time of supernatant harvesting from the G418-selected populations, and on TE671 cells after selection and pooling.

Article Snippet: PG13 gibbon ape leukemia virus (GaLV)-pseudotyped retroviral vector packaging cells39 were obtained from ATCC (Manassas, VA) and TE671 human rhabdomyosarcoma cells40 were a kind gift of Dr BA Bunnell (NHGRI, Bethesda, MD).

Techniques: Flow Cytometry, Expressing, Fluorescence, Transfection, Transduction, Selection